Title:


Measurement of alpha-Amilase Enzymes in Bacillus subtilis Bacteria


Author:


Mail Sukmawati Sukmawati(1*)
Mail Melda Yunita(2)
Mail Febrianti Rosalina(3)

(1) Faculty of Fisheries, Universitas Muhammadiyah Sorong, Indonesia
(2) Faculty of Medicine, Universitas Pattimura, Indonesia
(3) Faculty of Agriculture, Universitas Muhammadiyah Sorong, Indonesia
(*) Corresponding Author
10.31002/ijobe.v3i2.3237| Abstract views : 0 | PDF views : 0

Abstract


The bacterium Bacillus subtilis is able to produce amylase enzymes which are able to hydrolyze various types of starch sources into simple compounds such as maltose, glucose. Amylase converts carbohydrates which are polysaccharides into maltose. Amylase enzyme is a group of enzymes that are needed in the industrial field. The purpose of this study was to determine the level of the amylase enzyme produced by the bacterium Bacillus subtilis. The method used in this research is a quantitative method using an amylase enzyme measurement experiment. The results of this study are from two types of enzyme sources, namely Bacillus subtilis + Starch + NB and Bacillus subtilis + NB. Both samples showed enzyme activity. The conclusion of this study is that Bacillus subtilis + Starch + NB produces 63.186 units of the alpha-amylase enzyme used to produce 1 ppm / 1 micromol maltose per minute. Whereas the Bacillus subtilis + NB sample was 19.474 units of the alpha-amylase enzyme used to produce 1 ppm / 1 micromol maltose per minute.

Keywords


alpha-Amilase Enzymes; Bacillus subtilis; Bacteria

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References


Ningsih, D. R., Rastuti, U., & Kamaludin, R. (2012). Karakterisasi enzim amilase dari bakteri Bacillus amyloliquefaciens. Prosiding, 3(1).

Craigen, B., Dashiff, A., & Kadouri, D. E. (2011). The use of commercially available alpha-amylase compounds to inhibit and remove Staphylococcus aureus biofilms. The open microbiology journal, 5, 21.

Kumaunang, M., & Kamu, V. (2011). Aktivitas enzim bromelin dari ekstrak kulit nenas (Anenas comosus). Jurnal ilmiah sains, 11(2), 198-201.

Dini, I. R., & Munifah, I. (2014). Produksi dan Karakterisasi Enzim Selulase Ekstrak Kasar dari Bakteri yang Diisolasi dari Limbah Rumput Laut. Jurnal Teknologi dan Industri Pertanian Indonesia, 6(3).

Angraini, E., Angraeni, D. N., Umami, S. S., Sumiati, E., & Taufiqurokhman, T. (2019, February). Antagonism of Lentinus Cladopus Lc4 Extract, Trichoderma sp. Jpa Extract on Bacillus sp., Xanthomonas sp. and E. Coli. In Journal of Physics: Conference Series (Vol. 1155, No. 1, p. 012057). IOP Publishing.

Hardianti, F., & Aziz, I. R. (2019). Identification of Pathogenic Bacteria on the Salted Fish Lutjanus Vivanus in Sorong City of West Papua. Malaysian Journal of Microbiology, 15(3), 237-244.

Pangesti, N. W. I., Pangastuti, A., & Retnaningtyas, E. (2012). Pengaruh penambahan molase pada produksi enzim xilanase oleh fungi Aspergillus niger dengan substrat jerami padi. Bioteknologi Biotechnological Studies, 9(2), 41-48.

Sukmawati, S., & Rosalina, F. (2020). Isolasi Bakteri Dari Tanah Sebagai Penghasil Senyawa Antimikrob. Biospecies, 13(1), 46-51.

Sukmawati, S. (2018). Isolasi Bakteri Selulolitik dari Limbah Kulit Pisang. Biotropic: The Journal of Tropical Biology, 2(1), 46-52.

Gunam, I. B. W., Buda, K., & Guna, I. M. Y. S. (2010). Pengaruh perlakuan delignifikasi dengan larutan NaOH dan konsentrasi substrat jerami padi terhadap produksi enzim selulase dari aspergillus niger NRRL A-II, 264. Jurnal Biologi Udayana, 14(2).

Sukmawati, S., & Badaruddin, M. I. (2019). Screening of Probiotic Bacteria Candidates in The Mangrove Tourism Area in Klawalu Sorong City West Papua. Bioscience, 3(2), 161-168.

Supriyanti, F. M. T., & Heryanto, T. E. (2013). Penentuan Aktivitas Amilase Kasar Termofil Bacillus subtilis Isolat Kawah Gunung Darajat Garut, Jawa Barat. Bionatura, 15(2).




DOI: http://dx.doi.org/10.31002/ijobe.v3i2.3237

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