Physiological Response on Broiler Chicken’s Liver Supplemented Amino Acid Metionine-Cystine in Feed Contaminated with Aflatoxin B1

Listya Purnamasari, Ali Agus, Cuk Tri Noviandi

Abstract


               The content of AFB1 in feed with low levels and spend a long time, will cause primary damage or primary liver carcinoma. This study aims to reduce the toxicity of AFB1 with amino acid methionine-cystine supplementation in broiler chicken feed. This study used a 3 × 3 factorial design with methionine-cystine amino acid levels (M + C: 75, 100 and 125%) and AFB1 levels (0, 200, and 400 ppb). The variables collected were liver physiology, liver histopathology, SGPT levels, and SGOT levels. Observations of liver physiology showed that feed containing aflatoxin without methionine-cystine amino acid had a paler yellowish color (T4, T7 and T8). Pathological examination resulted that aflatoxicosis will attack the liver. Transition amino acid cystines in chicken feed contaminated with AFB1 did not occur in blood SGPT levels. Blood SGOT levels were highest at 21 days of age, namely T2 (M + C 100%) and T3 (M + C 125%) at AFB1 0 ppb which showed excess liver damage. The administration of methionine-cystine amino acids of 75, 100 and 125% in chicken feed contaminated with AFB1 0, 200 and 400 ppb consumed by broilers carries a risk of physiological and pathological damage to chicken liver.


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References


Ahmad, R. Z. 2009. Cemaran kapang pada pakan dan pangendaliannya. J. Litbang Pertanian. 28 (1) : 15 – 22.

Bammler, T. K., D.H. Slone, and D.L Eaton. 2000. Effects of dietary oltipraz and ethoxyquin on Aflatoxin B1 biotransformation in Non-Human Primates. J. Toxicol. Sci. 54. 30 – 41.

Devegowda, G. and T. N. K. Murthy. 2005. Mycotoxins : Their Effect in Poultry and Some Practical Solution. In The Mycotoxin Blue Book. Edited by D. E. Diaz. Nottingham, University Press : 25 – 55.

Karakilcik, A. Z., M. Zerin, O. Arslan, Y. Nazligul, and H. Vural. 2004. Effects of vitamin C and E on liver enzymes and biochemical parameters of rabbits exposed to aflatoxin B1. Vet Human Toxicol46(4): 190 – 192.

Maryam, R. 1996. Residu aflatoksin dan metabolitnya dalam daging dan hati ayam. Prosiding Temu Ilmiah nasional Bidang Veteriner. Bogor, 336 – 339.

Mattjik, A. A. dan Sumertajaya. 2002. Perancangan Percobaan Dengan Aplikasi SAS dan Minitab Jilid 1 Edisi Kedua. IPB Press, Bogor.

Nahm K. H. 1995. Possibilites for preventing mycotoxicosis in domistic fowl. J. Word's Poultry Sci. 51: 177 – 185.

Nugrahani, W. 2013. Konsumsi minuman beroksigen dan dampaknya terhadap performa saat berolahraga, profil lipid, glukosa darah dan SGOT/SGPT. Tesis. Program Studi Ilmu Pangan Fakultas Teknik Pertanian. Institut Pertanian Bogor.

Patriana, U. dan E. S. Pribadi. 1996. Studi mengenai sifat akumulasi aflatoksin pada organ jeroan ayam yang mendapatkan suntikan kortikosteroid dan antibiotik. Med. Vet. 3(1): 43 – 52.

Pestka, J. J. and A. T. Smolinski. 2005. Deoxynivalenol: Toxicology and potential effect on humans. J. Toxicol. Environ. Health Brit. Rev. 8 : 39 – 69.

Priosoeryanto, B. P., H. Huminto, S. Estuningsih, E. Harlina, dan R. Tiuria. 2002. Aflatoksikosis pada itik ras pedaging dan ras lokal di suatu peternakan di Cianjur, Jawa Barat. J Mikol. Kedok. Ind.3(1-2): 24 – 27.

Smela, M. E., S. S. Curier, E. A. Bailey, and J. M. Essingmann. 2001. The Chemistry and biology of aflatoksin B1. Carcinogenesis 22 (4):535 – 545.

Sugito, W. Manulu, D. A. Astuti, E. Handharyani, dan Chairul. 2006. Histopatologi hati dan ginjal pada ayam broiler yang dipapar cekaman panas dan diberi ekstrak kulit batang jaloh (Salix tetrasperma roxb). Semnas. Teknol. Peternakan dan Vet: 728 – 734.

Susanto, A. 2014. Glukomanan dari iles-iles (Amorphopalus ancophylus) sebagai pengikat aflatoksin pada pakan ayam broiler. Disertasi. Fakultas Peternakan. Institut Pertanian Bogor, Bogor.

Talwar, G. P. and L. M. Srivastava. 2004. Textbook of Biochemistry And Human Biology. 3rd Ed. Prentice-Hall Pvt.Ltd, India.

Transito, L. S., J. C. D. Rio-Garcia, J. L. Arjona-Roman, E. Moreno-Martinez, and A. Mendez-Albores. 2011. Effect of citric acid supplemented diets on aflatoxin degradation growth performance and serum parameters in broiler chickens. Arch. Med. Vet. 43: 215 – 222.

Utami, M. M. D., 2009. Efektivitas Ekstrak Bawang Putih dalam Pakan untuk Detoksifikasi Aflatoksin B1 pada Ayam Broiler. Tesis. Fakultas Peternakan. Universitas Gadjah Mada. Yogyakarta.

Valvida, A. G., A. Martinez, F. J. Damian, T. Quezda, R. Ortiz, C. Martinez, J. Lamas, L. Rodriguez, L. Yamamoto, F. Jaramillo, M.G. Loarca-Pina, and J. L. Reyes. 2001. Efficacy of N-Acetylcysteine to reduce the effects of Aflatoxin B1 Intoxication in Broiler Chicks. J. Poultry Sci. 80: 727 – 734.

Widiastuti, R. 2014. Residu aflatoksin dan metabolitnya pada berbagai produk pangan asal hewan dan pencegahannya. Wartazoa 24 (3): 179 – 190.

Yunianta. 2013. Upaya Penurunan Tingkat Toksisitas Aflatoksin B1 pada Jagung serta Penggunaannya Sebagai Pakan Broiler. Tesis. Fakultas Peternakan. Universitas Gadjah Mada, Yogyakarta.

Yunus, A. W., E. Razzazi-Fazeli, and J. Bohm. 2011. Aflatoxin B1 in affecting Broiler’s performance, immunity, and gastrointestinal tract: A review of history and contemporary issues. J. Toxins 3: 566 – 590.




DOI: 10.31002/jalspro.v3i1.1375

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