Title:


INDUKSI TUNAS MIKRO PISANG KULTIVAR AMBON NANGKA (Musa sp.) SECARA IN VITRO


Author:


Mail Rendie Prasetyo(1*)
Mail Sugiyono Sugiyono(2)
Mail Lucky Prayoga(3)

(1) Universitas Jenderal Soedirman, Indonesia
(2) , Indonesia
(3) , Indonesia
(*) Corresponding Author
10.31002/vigor.v5i2.3044| Abstract views : 0 | PDF views : 0

Abstract


The research was carried out to determne the best concentration of kinetin and activated charcoal to stimulate the shoot growth of Ambon Nangka banana cultivar. This research used a Completely Randomised Design (CRD) arranged factorials. The first factor was the concentration of kinetin with 4 levels i.e. 15 µM, 20 µM, 25 µM and 30 µM. The second factor was the concentration of activated charcoal with 4 levels i.e. 0 gL-1; 1 gL-1; 2 gL-1; and 3 gL-1. Each treatment combination was repeated 3 times, which resulted in 48 experimental units. The parameters measured were the shoot emergence time, number of shoots, shoot length, number of roots and root length. The data were analyzed using an analysis of variance at 95% and 99% level of confidences. The research results showed that the interaction between kinetin and activated charcoal had no effect on shoot length of Ambon Nangka banana cultivar. It was also found that the addition of 20 µM kinetin without any added activated charcoal resulted in the highest shoot number of Ambon Nangka banana cultivar.

Keywords: Ambon Nangka, kinetin, activated charcoal


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References


Abidin, Z. (1990). Dasar-dasar Pengetahuan tentang Zat Pengatur Tumbuh. Angkasa.

Ali, A., Sajid, A., Naveed, N. H., Majid, A., Saleem, A., Khan, U. A., Jafery, F. I., dan Naz, S. (2011). Initiation, proliferation and development of micropropagation system for mass scale production of banana through meristem culture. African Journal of Biotechnology. https://doi.org/10.5897/AJB11.2079

Balosi, F., Lakani, I., dan Panggeso, J. (2014). Pengendalian Hayati Terhadap Penyakit Darah Pada Tanaman Pisang Secara in-Vitro. E-J. Agrotekbis.

Damasco, O. P., Adkins, S. W., Godwin, I. D., dan Smith, M. K. (1998). Use of a SCAR-based marker for the early detection of dwarf off-types in micropropagated Cavendish bananas. Acta Horticulturae. https://doi.org/10.17660/ActaHortic.1998.461.15

Damayantu, F., dan Samurianto. (2010). Konservasi In Vitro plasma nutfah pisang untuk aplikasi di bank gen. Bioprodpek, 7(11), 86–91.

Edison, H. S., Susanto, A., Hermanto, C., dan Harahap, D. (2001). Karakterisasi beberapa sifat genotype plasma nutfah pisang. Buletin Plasma Nutfah. Balai Penelitian dan Pengembangan Pertanian.

El-Showk, S., Ruonala, R., dan Helariutta, Y. (2013). Crossing paths: Cytokinin signalling and crosstalk. In Development (Cambridge). https://doi.org/10.1242/dev.086371

Gunawan, L. W. (1992). Teknik Kultur Jaringan. Laboratorium Kultur Jaringan Tanaman Pusat Bioteknologi.

Halperin, W. (1978). Alternative morphogenic events in cells suspensions. American Jounal Bot, 53, 443–453.

Hartoyo. (1988). Classification of charcoal in fluidized bed for manufacturing active carbon. Jurnal Penelitian Hasil Hutan, 5(1), 17–22.

Heddy, S. (1989). Hormon Tumbuhan. CV Rajawali.

Huang, X., Huang, X. L., Wang, H. H., dan Li, X. J. (2001). Studies on the plant regeneration from the micro-cross sections of banana. Acta Horticulturae, 28, 19–24.

Müller, D., Waldie, T., Miyawaki, K., To, J. P. C., Melnyk, C. W., Kieber, J. J., Kakimoto, T., dan Leyser, O. (2015). Cytokinin is required for escape but not release from auxin mediated apical dominance. Plant Journal. https://doi.org/10.1111/tpj.12862

Rahardja, P. C. (1988). Kultur Jaringan. Teknik Perbanyakan Tanaman Secara Modern. Penebar Swadaya.

Salisbury, F. B., dan Ross, C. W. (1995). Fisiologi Tumbuhan. ITB.

Schaller, G. E., Street, I. H., dan Kieber, J. J. (2014). Cytokinin and the cell cycle. Current Opinion in Plant Biology, 21, 7–15. https://doi.org/10.1016/j.pbi.2014.05.015

Soesanto, L., dan Rahayuniati, R. F. (2009). Pengimbasan Ketahanan Pisang terhadap Layu Fusarium dengan Jamur Antagonis. Jurnal Hama Dan Penyakit Tumbuhan Tropika. https://doi.org/10.23960/J.HPTT.29130-140

Subramanyam, K., Subramanyam, K., Sailaja, K. V., Srinivasulu, M., dan Lakshmidevi, K. (2011). Highly efficient Agrobacterium-mediated transformation of banana cv. Rasthali (AAB) via sonication and vacuum infiltration. Plant Cell Reports. https://doi.org/10.1007/s00299-010-0996-4

Sumartini. (2012). Penyakit tular tanah (Sclerotium rolfsii dan Rhizoctonia solani) pada tanamn kacang-kacangan dan umbi-umbian serta cara pengendaliannya. Jurnal Litbang Pertanian.

Suyanti, S., dan Supriyadi, A. (2008). Pisang Budidaya, Pengolahan dan prospek Pasar. Jurnal Hasil Penelitian Program Study Keteknikan Pertanian.

Tanaka, M., Takei, K., Kojima, M., Sakakibara, H., dan Mori, H. (2006). Auxin controls local cytokinin biosynthesis in the nodal stem in apical dominance. Plant Journal. https://doi.org/10.1111/j.1365-313X.2006.02656.x

Vishnevetsky, J., White, T. L., Palmateer, A. J., Flaishman, M., Cohen, Y., Elad, Y., Velcheva, M., Hanania, U., Sahar, N., Dgani, O., dan Perl, A. (2011). Improved tolerance toward fungal diseases in transgenic Cavendish banana (Musa spp. AAA group) cv. Grand Nain. Transgenic Research. https://doi.org/10.1007/s11248-010-9392-7

Wareing, P. F., dan Philips, I. D. J. (1981). The Control of Growth and Differentiation in Plants. Pergamon Press.

Wattimena, G. A. (1988). Zat Pengatur Tumbuh Tanaman. Pusat Antar Universitas Pertanian Bogor.

Widiastoeti, D., Santi, A., dan Solvia, N. (2013). Pengaruh Myoinositol dan Arang Aktif terhadap Pertumbuhan Planlet Anggrek Dendrobium dalam Kultur In Vitro. Jurnal Hortikultura. https://doi.org/10.21082/jhort.v22n3.2012.p205-209

Wijayanti, A. (2002). Pengaruh Bahan Eksplan Terhadap Pertumbuhan Melati (Jasminum sambac Ait) secara In vitro. Agrivet, 6(1), 13–22.

Zulkarnain. (2009). Kultur Jaringan Tanaman, Solusi Perbanyakan Tanaman Budi Daya. Bumi Aksara.




DOI: http://dx.doi.org/10.31002/vigor.v5i2.3044


DOI (PDF): http://dx.doi.org/10.31002/vigor.v5i2.3044.g1726

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