The Determination of Total Flavonoid Content and Test for Glucose Level Reduction in Parijoto Fruits Extract from Bandungan

Abstract

The variety of flora in Indonesia can be used as herbal medicine, one of which is the Parijoto fruit (Medinilla speciosa Blume). Parijoto fruit prospers in highland areas and looks like purple grapes. The purpose of this study was to determine the activity of reducing glucose levels and to determine the total flavonoid content of parijoto fruit extract from Bandungan. Extraction of secondary metabolites of parijoto fruits using maceration method, with 96% ethanol solvent. The activity of reducing glucose levels was tested in vitro using the Nelson-Somogyi method. The total flavonoid content was determined spectrophotometrically using AlCl₃ reagent with the results expressed in quercetin equivalents (µg QE/g extract). The results showed that parijoto fruits extract at concentrations of 10,20, 30, 40, 50, and 60 ppm was able to reduce glucose levels by 68.6419%, 53.2158%, 45.7885%, 52.3996%, 55.2563%, and 39.5854%. The total flavonoid content of parijoto fruit extract was 15.98 µg QE/g extract. These results mean that flavonoids in parijoto fruits extracted using 96% solvent have the potential to reduce glucose levels.

Keywords:   Parijoto, fruit, glucose levels, total flavonoids