Molecular Detection of White Spot Syndrome Virus (WSSV) and Taura Syndrome Virus (TSV) on Vaname Shrimp (Littopenaues Vannamei) Using Polymerase Chain Reaction (PCR) Method at BLUPPB Karawang

Abstract

Shrimp production numbers can decrease due to diseases caused viruses, bacteria or parasites. The purpose of this research  is to find out the mechanism and find obstacles in detecting TSV and WSSV diseases using the PCR method. The research has been carried out in the time period January 3 - February 3, 2024 which is located at BLUPPB Karawang. The number of samples was 15 shrimps which were selected randomly and divided into 3 groups, each containing 5 shrimps (codes W.01; W.02; and T.01). The data analysis method uses qualitative data analysis by examining the results of observations in visual form. The results showed that sample W.01 formed DNA bands between 630, 550, and 333 bp, sample W.02 formed bands between 630 and 550 bp, while sample T.01 formed RNA bands only at 476 bp. Efforts to implement biosecurity is a method that can be applied to prevent infection rates because no cure has been found for WSSV and TSV viruses that infect shrimp.

Keywords:  BLUPPB Karawang, PCR, TSV, Vaname shrimp, WSSV.